In this method, analytes were extracted using acetonitrile mixed with ammonia solution. After dehydration and evaporated to dryness, extracts were purified using an Oasis MAX cartridge. Chromatography separation was performed on a hydrophilic interaction liquid chromatography column. The analytes were then determined using triple-quadrupole mass spectrometry in positive electrospray ionization and multiple reaction monitoring mode. Calibration plots were constructed using matrix-matched standards and showed good linearity. Limits of quantification in all tissues for 4-methylaminoantipyrine(MAA),4-formylaminoantipyrine(FAA),and4-acetylaminoantipyrone (AAA) ranged from 0.4μg kg−1 to 6 μg kg−1, while that for 4-aminoantipyrone (AA) ranged from 10 μg kg−1 to 125 μg kg−1. Limits of detection for MAA, FAA, and AAA in all the tissues ranged from 0.08~1.2 μg kg−1, and limits of detection for AA ranged from 2~25 μg kg−1.The developed method was successfully applied in the determination of DIP metabolite residues in actual goat tissues.

The study is of great significance to guarantee animal husbandry develope smoothly，promote the export trade of agricultural products，keep human health and maintain the national food safety.

 

By Zhang Lifang
twinzhang@shvri.ac.cn