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WOX Protein LFL Mainly Acts as a Repressor in Regulating Flower Development
An international research team cooperated between Dr. Lifang Niu and Dr. Hao Lin from BRI, the elite youth program candidates of CAAS, and researchers in Oklahoma State University, USA revealed that the WUSCHEL related homeobox (WOX) protein LOOSE FLOWER (LFL) mainly acts as a transcriptional repressor in regulating flower development. This work was recently published online in The Plant Journal.
LFL mainly acts as a transcriptional repressor in flower development.
(a) Schematic representation of reporter and effector constructs used in transient expression assay. (b) Relative luciferase activities measured as bioluminescence in Arabidopsis protoplasts. Error bars indicates SD (n = 3). **P < 0.01 (t-test). (c-h) Floral organ phenotypes of lfl-1 mutant plants complemented with mutated LFL construct pLFL::LFLm (c, f), exogenous repressor domain fused to the mutant pLFL::SRDX-LFLm1 (d, g), and exogenous activator domain fused to the mutant pLFL::LFLm1-VP16 (e, h). Bars = 1mm.
Flowers, the reproductive structures of flowering plants, are one of the key factors influencing plant survival and grain yield. The WOX family genes play key roles in plant development including stem cell maintenance, embryonic patterning, and lateral organ development. However, the molecular mechanism of these actions remains to be elucidated. In this study, researchers characterized a loose flower mutant, lfl, which is defective in floral organ fusion, by screening Tnt1 retrotransposon-tagged lines of M. truncatula. LFL encodes a WOX domain transcription factor and LFL mainly acts as a transcriptional repressor through its WUS-box in regulating floral organ development. Besides, LFL can physically interact with the transcriptional co-repressor TOPLESS (Mt-TPL), indicating that repression is central to LFL function, and Mt-TPL is a key component of this repressive activity. These works revealed a repressive mechanism of WOX protein LFL through recruiting co-repressor Mt-TPL, which provide a new sight into understanding the mechanistic functions of WOX genes in regulating flower organ development.
Dr. Lifang Niu and Dr. Hao Lin are the Co-first authors of this paper and this work was supported by The Elite Youth Program of CAAS.
More details are available on the bellow links: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12743/abstract
(a) Schematic representation of reporter and effector constructs used in transient expression assay. (b) Relative luciferase activities measured as bioluminescence in Arabidopsis protoplasts. Error bars indicates SD (n = 3). **P < 0.01 (t-test). (c-h) Floral organ phenotypes of lfl-1 mutant plants complemented with mutated LFL construct pLFL::LFLm (c, f), exogenous repressor domain fused to the mutant pLFL::SRDX-LFLm1 (d, g), and exogenous activator domain fused to the mutant pLFL::LFLm1-VP16 (e, h). Bars = 1mm.
Flowers, the reproductive structures of flowering plants, are one of the key factors influencing plant survival and grain yield. The WOX family genes play key roles in plant development including stem cell maintenance, embryonic patterning, and lateral organ development. However, the molecular mechanism of these actions remains to be elucidated. In this study, researchers characterized a loose flower mutant, lfl, which is defective in floral organ fusion, by screening Tnt1 retrotransposon-tagged lines of M. truncatula. LFL encodes a WOX domain transcription factor and LFL mainly acts as a transcriptional repressor through its WUS-box in regulating floral organ development. Besides, LFL can physically interact with the transcriptional co-repressor TOPLESS (Mt-TPL), indicating that repression is central to LFL function, and Mt-TPL is a key component of this repressive activity. These works revealed a repressive mechanism of WOX protein LFL through recruiting co-repressor Mt-TPL, which provide a new sight into understanding the mechanistic functions of WOX genes in regulating flower organ development.
Dr. Lifang Niu and Dr. Hao Lin are the Co-first authors of this paper and this work was supported by The Elite Youth Program of CAAS.
More details are available on the bellow links: http://onlinelibrary.wiley.com/doi/10.1111/tpj.12743/abstract
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