The Agro-product Quality and Safety Testing Technology Innovation Team from the Institute of Quality Standards and Testing Technology for Agro-Products of CAAS has made considerable progress in recombinant full-length antibody against the pesticide ethirimol, successfully achieving the “immortalization” of the antibodies. Relevant results were published in the Journal of Hazardous Materials (IF: 13.6).

In this study, anti-ethirimol monoclonal antibody was prepared using a rational hapten design strategy assisted by chemical calculation. The variable region gene of the monoclonal antibody was further decoded and recombined. The full-length antibody was efficiently expressed by a mammalian expression system. Notably, the recombinant full-length antibody exhibited performance that was highly congruent with its parental monoclonal counterpart. Furthermore, a comprehensive analysis of the high-affinity recognition mechanism of the recombinant full-length antibody was conducted using homology modeling and molecular simulation techniques. This analysis elucidated the crucial amino acid site responsible for the antibody's recognition of the pesticide ethirimol, serving as a theoretical foundation for future in vitro evolution of the antibody.

These research outcomes have successfully addressed the challenges associated with the positive loss of monoclonal cell lines, batch-to-batch instability, and the stringent storage conditions required for cell lines, typically maintained at approximately -200℃. To ensure the consistency and stability of the antibody, it suffices to merely store the antibody sequence within the computer system. This innovation offers novel insights and solutions for the efficient preparation and reliable application of antibodies.

This research was supported by the National Natural Science Foundation of China (32272423) and the Central Public Interest Scientific Institution Basal Research Fund of the Chinese Academy of Agricultural Sciences (Y2021PT05). The master student Xingmei Lei is the first author of this paper, and postdoctoral student Peipei Li and Researcher Maojun Jin are the corresponding authors.

Link: https://doi.org/10.1016/j.jhazmat.2024.134067

Figure1 Preparation, characterization and application of recombinant full-length antibody against ethirimol            

By Maojun Jin (jinmaojun@caas.cn)