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Advances in Rapid Detection Sensor Research for Staphylococcus Aureus by the Smart Animal Husbandry Innovation Team

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Source : Institute of Animal Sciences

Staphylococcus aureus (SA) poses a serious risk to human and animal health, necessitating a low-cost and high-performance analytical platform for point-of-care diagnostics. Herein, Smart Animal Husbandry Innovation Team of IAS proposed a controllable self-cleaning FET to simplify (bio)chemical modification and improve mechanical stability and anti-fouling performance, and then constructed an RCD based DNA nanotree to significantly enhance the sensitivity for SA detection. An RCD-based DNA nanotree was designed and synthesized based on similar DNA-origami technology to improve the sensitivity. The controllable self-cleaning FET biosensor was applied for SA detection without cultivation, which had a wide linear range from 1 to 105 CFU/mL with low detection of 1 CFU/mL. This achievement provides a new method for developing low-cost, high-performance biosensors in the livestock industry.The findings are published in the Journal of Nanobiotechnology.

This work is supported by the National Key Research and Development Program, the State Key Laboratory of Animal Nutrition and Feeding, among other projects.

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https://doi.org/10.1186/s12951-024-02682-3 

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